爱泼斯坦-巴尔病毒DNA在淋巴母细胞系RGN1中通过潜伏复制起点与人类基因组发生重组。

Gualandi G, Santolini E, Calef E

Department of Biology, II University of Rome, Italy.

J Virol. 1992 Sep;66(9):5677-81. doi: 10.1128/JVI.66.9.5677-5681.1992.

We show here that in a lymphoblastoid cell line Epstein-Barr virus DNA recombines with the human genome. The genetic exchange involves the oriP region of the virus. A junction between viral and human DNA from this line has been cloned and sequenced. The results indicate that the integration of Epstein-Barr virus DNA involves a region of the human genome which contains internal short repetition. An 800-bp probe has been isolated from the human part of the junction. This probe has been used to show that the human region exists as a duplication in normal cells.

我们在此表明，在一个淋巴母细胞系中，爱泼斯坦 - 巴尔病毒DNA与人类基因组发生重组。这种基因交换涉及病毒的oriP区域。来自该细胞系的病毒DNA与人类DNA之间的一个连接点已被克隆并测序。结果表明，爱泼斯坦 - 巴尔病毒DNA的整合涉及人类基因组中一个包含内部短重复序列的区域。已从连接点的人类部分分离出一个800碱基对的探针。该探针已被用于表明该人类区域在正常细胞中以重复形式存在。