Kündig T M, Althage A, Hengartner H, Zinkernagel R M
Laboratory of Experimental Immunology, University of Zurich, Switzerland.
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7757-61. doi: 10.1073/pnas.89.16.7757.
A way to assess specific CD8+ T-cell activity in a skin test analogous to the conventional delayed-type hypersensitivity (DTH) reaction for CD4+ T cells is presented. Local injection of viral class I binding peptides caused a specific CD8+ T-cell-mediated DTH in footpads of virally infected mice. The DTH was inducible only during the acute phase of the infection. Apparently because of the short half-life of locally available peptide, only activated CD8+ effector T cells could mediate the reaction. This skin test may prove to be particularly interesting for use in humans to evaluate the activation status of CD8+ T cells during acute viral infections and of memory CD8+ T cells, for example, in chronically active immunopathological disease or infection.
本文提出了一种在皮肤试验中评估特异性CD8 + T细胞活性的方法，该方法类似于针对CD4 + T细胞的传统迟发型超敏反应（DTH）。在病毒感染小鼠的足垫中，局部注射病毒I类结合肽可引发特异性CD8 + T细胞介导的DTH。这种DTH仅在感染的急性期可诱导产生。显然，由于局部可用肽的半衰期较短，只有活化的CD8 +效应T细胞能够介导该反应。这种皮肤试验可能被证明在人类中特别有趣，可用于评估急性病毒感染期间CD8 + T细胞的活化状态以及记忆CD8 + T细胞的活化状态，例如在慢性活动性免疫病理疾病或感染中。
