Gauquelin G, Maillet A, Allevard A M, Vorobiev D, Grigoriev A I, Gharib C
Laboratoire de Physiologie de l'Environment, Faculté de Médecine Grange-Blanche, Lyon, France.
Eur J Appl Physiol Occup Physiol. 1992;65(1):25-9. doi: 10.1007/BF01466270.
Using a specific and sensitive radio-immunoassay involving separation and extraction procedures, we measured the concentration of saliva and plasma atrial natriuric factor (ANF) and cyclic guanosine monophosphate (cGMP) in men before and during a 10 h head-down tilt at -6 degrees. Saliva values closely correlated with plasma for ANF (r = 0.7-0.95) and for cGMP (r = 0.65-1). During this dynamic test, the mean concentrations of ANF and cGMP were significantly higher after 15 and 45 min, respectively, this increase persisting for 3.5 h. We concluded that the concentration of ANF in saliva may be significantly affected by a marked fluid shift from the lower to the upper half of the body. This is the first time that the presence of ANF and cGMP has been demonstrated in saliva. The great advantage of studying saliva is that it can be obtained non-invasively in athletes or during space flight. This methodology will be used during the Soviet-French space flight (Antares Project) planned for 1992.
我们采用一种涉及分离和提取程序的特异性灵敏放射免疫分析法，测量了男性在-6度头低位倾斜10小时之前及期间唾液和血浆中的心钠素（ANF）及环磷酸鸟苷（cGMP）的浓度。唾液中ANF值（r = 0.7 - 0.95）及cGMP值（r = 0.65 - 1）与血浆中的值密切相关。在这个动态试验中，ANF和cGMP的平均浓度分别在15分钟和45分钟后显著升高，且这种升高持续3.5小时。我们得出结论，从身体下半部到上半部的明显体液转移可能会显著影响唾液中ANF的浓度。这是首次在唾液中证实存在ANF和cGMP。研究唾液的一大优势在于，它可以在运动员或太空飞行期间以非侵入性方式获取。这种方法将用于计划于1992年进行的苏联 - 法国太空飞行（安塔瑞斯项目）。
