Gerbes A L, Arendt R M, Gerzer R, Schnizer W, Jüngst D, Paumgartner G, Wernze H
Medizinische Klinik II, Klinikum Grosshadern, University of Munich, FRG.
Eur J Clin Invest. 1988 Aug;18(4):425-9. doi: 10.1111/j.1365-2362.1988.tb01035.x.
The role of the atrial natriuretic factor (ANF), its second messenger cyclic guanosine monophosphate (cGMP), and the counteracting renin-aldosterone system in acute volume regulation was investigated in 25 healthy human subjects. Central volume stimulation by 1-h head-out water immersion (WI) into a thermoneutral water-bath increased plasma levels of ANF (mean +/- SEM) from 6.0 +/- 0.6 to 13.6 +/- 2.6 fmol ml-1. This was paralleled by a rise of plasma cGMP levels from 1.9 +/- 0.2 to 2.8 +/- 0.4 pmol ml-1, and an increase of urinary cGMP excretion from 340 +/- 64 to 692 +/- 103 pmol min-1. Water immersion reduced plasma aldosterone concentration (PAC) from 13.0 +/- 1.7 to 6.5 +/- 0.8 ng 100 ml-1 and plasma renin activity (PRA) from 5.3 +/- 0.9 to 2.4 +/- 0.3 ng AI ml-1 h-1. Volume stimulation markedly increased diuresis and natriuresis. Whereas the plasma cGMP increase correlated with plasma ANF stimulation, neither ANF nor PRA or PAC correlated with basal or stimulated renal parameters. Water immersion-induced changes in natriuresis and urinary cGMP excretion were correlated. These data suggest a role of ANF and cGMP in acute volume regulation of healthy human subjects.
在25名健康人体受试者中研究了心房利钠因子（ANF）、其第二信使环磷酸鸟苷（cGMP）以及起拮抗作用的肾素 - 醛固酮系统在急性容量调节中的作用。通过在热中性水浴中进行1小时头低位水浸（WI）来刺激中心容量，可使ANF的血浆水平（平均值±标准误）从6.0±0.6飞升/毫升升至13.6±2.6飞升/毫升。与此同时，血浆cGMP水平从1.9±0.2皮摩尔/毫升升至2.8±0.4皮摩尔/毫升，尿cGMP排泄量从340±64皮摩尔/分钟增至692±103皮摩尔/分钟。水浸使血浆醛固酮浓度（PAC）从13.0±1.7纳克/100毫升降至6.5±0.8纳克/100毫升，血浆肾素活性（PRA）从5.3±0.9纳克血管紧张素I/毫升·小时降至2.4±0.3纳克血管紧张素I/毫升·小时。容量刺激显著增加了利尿和利钠作用。虽然血浆cGMP的增加与血浆ANF的刺激相关，但ANF、PRA或PAC与基础或刺激后的肾脏参数均无相关性。水浸引起的利钠作用变化与尿cGMP排泄相关。这些数据表明ANF和cGMP在健康人体受试者的急性容量调节中起作用。
