Gerzer R, Witzgall H, Tremblay J, Gutkowska J, Hamet P
J Clin Endocrinol Metab. 1985 Dec;61(6):1217-9. doi: 10.1210/jcem-61-6-1217.
We studied the effects of a bolus injection of 50/micrograms synthetic human atrial natriuretic factor (ANF) on the cyclic GMP and cyclic AMP levels in plasma and urine of eight normal men. Administration of the hormone increased basal immunoreactive (IR-) ANF levels in plasma 2.8-fold to 110 pM three minutes after injection. Thereafter, IR-ANF levels rapidly declined to basal levels. Plasma cyclic GMP levels increased 2.6-fold to 16.6 nM within 6 minutes after ANF and decreased to near basal values within 30 minutes. Urinary cyclic GMP excretion increased 2.8-fold, whereas urinary volume and sodium excretion increased less than two-fold in the 30 minutes after ANF. Plasma cyclic AMP levels did not change. The data indicate that changes in plasma IR-ANF levels are followed by changes in plasma cyclic GMP and in urinary cyclic GMP excretion and suggest that cyclic GMP is a biological marker for circulating ANF in man.
我们研究了对8名正常男性静脉推注50微克合成人心房利钠因子（ANF）后，其血浆和尿液中环鸟苷酸（cGMP）及环腺苷酸（cAMP）水平的变化。注射该激素后3分钟，血浆中基础免疫反应性（IR-）ANF水平升高2.8倍，至110皮摩尔/升。此后，IR-ANF水平迅速降至基础水平。ANF注射后6分钟内，血浆cGMP水平升高2.6倍，至16.6纳摩尔/升，并在30分钟内降至接近基础值。ANF注射后30分钟内，尿cGMP排泄量增加2.8倍，而尿量和钠排泄量增加不到两倍。血浆cAMP水平未发生变化。这些数据表明，血浆IR-ANF水平变化后，血浆cGMP和尿cGMP排泄量也会随之改变，提示cGMP是人体循环中ANF的生物学标志物。
