活性菠菜乙醇酸氧化酶在大肠杆菌中的直接表达。
Direct expression of active spinach glycolate oxidase in Escherichia coli.
作者信息
Macheroux P, Mulrooney S B, Williams C H, Massey V
机构信息
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor.
出版信息
Biochim Biophys Acta. 1992 Aug 17;1132(1):11-6. doi: 10.1016/0167-4781(92)90046-3.
Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expressed in E. coli has identical physico-chemical features to the wild-type enzyme, but is expressed at a level approx. 15-fold higher than in the yeast system.
菠菜乙醇酸氧化酶（GAO）利用T7 RNA聚合酶启动子在大肠杆菌中表达。该酶约占可溶性蛋白组分的1%，以可溶性活性酶的形式表达。与在酿酒酵母中表达的GAO（Macheroux, P., Massey, V., Thiele, D.J. 和Volokita, M. (1991) Biochemistry 30, 4612 - 4619）相比，结果表明在大肠杆菌中表达的GAO与野生型酶具有相同的物理化学特性，但表达水平比酵母系统中高约15倍。
Zotero 插件