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一种三共振脉冲序列，用于使酰胺1HN和15N核与前一个残基的1Hα质子选择性相关。

A triple-resonance pulse scheme for selectively correlating amide 1HN and 15N nuclei with the 1H alpha proton of the preceding residue.

作者信息

Clubb R T, Wagner G

机构信息

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.

出版信息

J Biomol NMR. 1992 Jul;2(4):389-94. doi: 10.1007/BF01874816.

DOI:10.1007/BF01874816
PMID:1324757
Abstract

A 3D 1H-15N-13C triple resonance experiment is presented that contains exclusively cross peaks between the 1HN and 15N nuclei of one residue with the H alpha of the preceding residue. The pulse sequence, designed to minimize the time coherence, is transverse on nuclei with short T2 values. The experiment consists of coherence transfers via one-bond couplings from the HN via N, CO, C alpha to the H alpha and back to the HN for detection; it is called HN(COCA)HA. The experiment was tested on uniformly 15N- and 13C-enriched T4 lysozyme.

摘要

本文介绍了一种3D 1H-15N-13C三重共振实验，该实验仅包含一个残基的1HN和15N核与前一个残基的Hα之间的交叉峰。为了使时间相干最小化而设计的脉冲序列，对于具有短T2值的核是横向的。该实验包括通过单键耦合从HN经由N、CO、Cα到Hα的相干转移，然后再回到HN进行检测；它被称为HN(COCA)HA。该实验在均匀15N和13C富集的T4溶菌酶上进行了测试。

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本文引用的文献

1
Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.相敏二维相关光谱法（COSY）在蛋白质中¹H-¹H自旋-自旋耦合常数测量中的应用。
Biochem Biophys Res Commun. 1983 Jun 29;113(3):967-74. doi: 10.1016/0006-291x(83)91093-8.
2
Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor.蛋白质 1H 核磁共振谱中的序列共振归属。碱性胰蛋白酶抑制剂。
J Mol Biol. 1982 Mar 5;155(3):347-66. doi: 10.1016/0022-2836(82)90009-2.
3
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.
Disorder and structure in the Rab11 binding domain of Rab11 family interacting protein 2.
Rab11家族相互作用蛋白2的Rab11结合结构域中的无序与结构
Biochemistry. 2009 Jan 27;48(3):549-57. doi: 10.1021/bi8020197.
4
A tracked approach for automated NMR assignments in proteins (TATAPRO).蛋白质中自动核磁共振归属的跟踪方法（TATAPRO）。
J Biomol NMR. 2000 Jun;17(2):125-36. doi: 10.1023/a:1008315111278.
5
High-resolution detection of five frequencies in a single 3D spectrum: HNHCACO--a bidirectional coherence transfer experiment.在单个三维谱中对五个频率进行高分辨率检测：HNHCACO——一种双向相干转移实验。
J Biomol NMR. 1998 Feb;11(2):185-90. doi: 10.1023/a:1008229723544.
6
Automated probabilistic method for assigning backbone resonances of (13C,15N)-labeled proteins.用于确定（13C，15N）标记蛋白质主链共振的自动概率方法。
J Biomol NMR. 1997 Feb;9(2):151-66. doi: 10.1023/a:1018602220061.
7
Phase labeling of C-H and C-C spin-system topologies: application in constant-time PFG-CBCA(CO)NH experiments for discriminating amino acid spin-system types.
J Biomol NMR. 1996 Oct;8(3):345-50. doi: 10.1007/BF00410332.
8
3D triple-resonance NMR techniques for the sequential assignment of NH and 15N resonances in 15N- and 13C-labelled proteins.用于对15N和13C标记蛋白质中的NH和15N共振进行序列归属的3D三共振核磁共振技术。
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9
Resonance assignment strategies for the analysis of NMR spectra of proteins.用于分析蛋白质核磁共振谱的共振归属策略。
Mol Biotechnol. 1994 Aug;2(1):61-93. doi: 10.1007/BF02789290.
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4
Assignment of the backbone 1H and 15N NMR resonances of bacteriophage T4 lysozyme.噬菌体T4溶菌酶主链1H和15N核磁共振共振峰的归属
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5
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6
A new 3D HN(CA)HA experiment for obtaining fingerprint HN-Halpha peaks in 15N- and 13C-labeled proteins.一种用于在15N和13C标记的蛋白质中获取指纹HN-Hα峰的新型3D HN(CA)HA实验。
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