Bende R J, Jochems G J, Frame T H, Klein M R, van Eijk R V, van Lier R A, Zeijlemaker W P
Department of Hybridoma and Tissue Culture, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Cell Immunol. 1992 Sep;143(2):310-23. doi: 10.1016/0008-8749(92)90028-n.
In the present study we investigated whether interleukin-4 (IL-4), IL-5, and IL-6 could enhance the efficiency of Epstein-Barr virus (EBV) transformation for the generation of specific human monoclonal antibody (HuMAb)-producing B-cell lines directed against erythrocyte Rhesus(D) antigen. In newly EBV-infected B cells, IL-4 and IL-6 caused a comparable enhancement of proliferation and of total IgG and IgA production. IL-6 showed a much stronger effect than IL-4 on IgM production, whereas IL-4 was unique in inducing IgE production. No stimulatory effects of IL-5 on either growth or Ig production were observed. Although addition of IL-6 resulted during the early phase after EBV infection in high numbers of Ag-specific antibody-producing wells, this did not result in an increased number of stable HuMAb-secreting cell lines. When the effects of cytokines were tested on established polyclonal EBV B cells, in a high cell density culture system, only IL-6 was able to enhance Ig secretion, while no effect could be demonstrated on proliferation. These studies substantiate that IL-6 is an important regulator of proliferation and Ig production, and that it acts at distinct stages after EBV infection, but does not increase the final overall recovery of Ag-specific EBV B-cell lines.
在本研究中，我们调查了白细胞介素-4（IL-4）、IL-5和IL-6是否能够提高爱泼斯坦-巴尔病毒（EBV）转化效率，以生成针对红细胞恒河猴（D）抗原的特异性人单克隆抗体（HuMAb）产生B细胞系。在新感染EBV的B细胞中，IL-4和IL-6对增殖以及总IgG和IgA产生具有相当的增强作用。IL-6对IgM产生的作用比IL-4强得多，而IL-4在诱导IgE产生方面是独特的。未观察到IL-5对生长或Ig产生有刺激作用。尽管在EBV感染后的早期阶段添加IL-6导致大量产生抗原特异性抗体的孔出现，但这并未导致稳定分泌HuMAb的细胞系数量增加。当在高细胞密度培养系统中对已建立的多克隆EBV B细胞测试细胞因子的作用时，只有IL-6能够增强Ig分泌，而对增殖未显示出作用。这些研究证实IL-6是增殖和Ig产生的重要调节因子，并且它在EBV感染后的不同阶段起作用，但不会增加抗原特异性EBV B细胞系的最终总体回收率。
