Shepherd P, Lunny D, Brookes R, Palmer T, McCance D
Department of Immunology, Guy's Hospital, UMDS, London, UK.
Scand J Immunol Suppl. 1992;11:69-74. doi: 10.1111/j.1365-3083.1992.tb01623.x.
The presence of human papillomavirus (HPV) types 6, 16 and 18 in cervical biopsies can be detected by an immunoperoxidase technique using type-restricted monoclonal antibodies raised against fusion proteins representing the L1 major capsid proteins of these three HPV types. In a retrospective study (n = 54) we have used these antibodies and biotinylated DNA probes of HPV 6, 16 and 18 to detect and type HPV in formalin-fixed material from the cervix. The biopsies were classified histologically into normals, wart infections without dysplasia, cervical intraepithelial neoplasia (CIN) and squamous cell carcinomas. Antibody staining showed that 22% of all CIN was positive for HPV 16 and 40% of cervical warts were positive for HPV 6, 16 and 18. There was no HPV capsid protein detected in the normals and squamous cell carcinomas using these antibodies, whereas 25% of the tumours were positive for HPV 16 by in situ hybridization. Sections of cervical warts and CIN positive for HPV types by in situ hybridization were also positive by antibody staining which suggests that both techniques are detecting replicating virus. We feel these two techniques complement each other in detection and typing of HPV in cervical biopsies from patients with active disease.
采用针对代表这三种人乳头瘤病毒（HPV）L1主要衣壳蛋白的融合蛋白产生的型特异性单克隆抗体，通过免疫过氧化物酶技术可检测宫颈活检组织中HPV 6、16和18型的存在。在一项回顾性研究（n = 54）中，我们使用这些抗体以及HPV 6、16和18的生物素化DNA探针，来检测宫颈福尔马林固定材料中的HPV并进行分型。活检组织经组织学分类为正常、无发育异常的疣感染、宫颈上皮内瘤变（CIN）和鳞状细胞癌。抗体染色显示，所有CIN中有22%的HPV 16呈阳性，40%的宫颈疣HPV 6、16和18呈阳性。使用这些抗体在正常组织和鳞状细胞癌中未检测到HPV衣壳蛋白，而通过原位杂交25%的肿瘤HPV 16呈阳性。原位杂交显示HPV阳性的宫颈疣和CIN切片抗体染色也呈阳性，这表明两种技术均能检测到正在复制的病毒。我们认为这两种技术在检测患有活动性疾病患者宫颈活检组织中的HPV及其分型方面可相互补充。
