Tanya V N, Greiner E C, Gibbs E P
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0633.
Vet Microbiol. 1992 Jul;32(1):1-14. doi: 10.1016/0378-1135(92)90002-b.
Based upon epidemiological evidence, Culicoides insignis Lutz is a probable biological vector of bluetongue viruses (BTV) in South Florida, the Caribbean Region and Central America. The vector potential of this species for BTV was evaluated in the laboratory in a series of experiments using insects caught in the field. Although there was great variation in the percentage of flies that fed from any one catch, it was demonstrated that C. insignis became infected after membrane feeding on a mixture of blood and virus. The infection rates ranged from 20 to 62.5%. Following intrathoracic inoculation, BTV replicated to high titres in C. insignis. Such flies were also shown to be capable of transmitting BTV to susceptible sheep and embryonated chicken eggs. This series of experiments provides the first conclusive evidence that C. insignis is a biological vector of bluetongue virus. This is the first proven vector of BTV in the neotropics.
基于流行病学证据，在南佛罗里达州、加勒比地区和中美洲，格氏库蠓（Culicoides insignis Lutz）可能是蓝舌病毒（BTV）的生物传播媒介。在实验室中，利用野外捕获的昆虫进行了一系列实验，评估了该物种传播BTV的可能性。尽管从任何一批捕获的昆虫中取食的苍蝇百分比差异很大，但已证明格氏库蠓在取食血液和病毒混合物后会被感染。感染率在20%至62.5%之间。经胸腔接种后，BTV在格氏库蠓中复制到高滴度。这些苍蝇还被证明能够将BTV传播给易感绵羊和鸡胚。这一系列实验提供了首个确凿证据，证明格氏库蠓是蓝舌病毒的生物传播媒介。这是新热带区首个经证实的BTV传播媒介。
