Yamamoto T, Moerschell R P, Wakem L P, Komar-Panicucci S, Sherman F
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642.
Genetics. 1992 Aug;131(4):811-9. doi: 10.1093/genetics/131.4.811.
Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucleotides. This differential effect was observed with oligonucleotides designed to make alterations at six different sites along the gene and was independent of the oligonucleotide sequence and length, number of mismatches and the host strain. Competition studies showed that antisense oligonucleotides did not inhibit transformation. Although the mechanism for this strand specificity is unknown, this difference was maintained even when CYC1 transcription was diminished to approximately 2% of the normal level.
用有义寡核苷酸和反义寡核苷酸直接转化酿酒酵母的Cyc1突变体，以研究两条基因组DNA链在转化中的作用。有义寡核苷酸产生的转化体比反义寡核苷酸多约20倍。在用设计用于在基因上六个不同位点进行改变的寡核苷酸时观察到这种差异效应，并且与寡核苷酸序列和长度、错配数以及宿主菌株无关。竞争研究表明反义寡核苷酸不抑制转化。虽然这种链特异性的机制尚不清楚，但即使CYC1转录减少到正常水平的约2%时，这种差异仍然存在。
