Yamada R, Suzuki T, Hashimoto M, Eto Y, Shiokawa K, Muramatsu M
Department of Biochemistry, Faculty of Medicine, Saitama Medical School, Japan.
Biochem Biophys Res Commun. 1992 Aug 31;187(1):79-85. doi: 10.1016/s0006-291x(05)81461-5.
A human promyelocytic cell line, HL-60, treated with activin/EDF was found to differentiate into monocyte/macrophage-like cells. This was shown not only by morphology but by the loss of myeloperoxidase granules and the appearance of nonspecific esterase. Dose-dependent inhibition of the differentiation by follistatin, an activin-binding protein, confirmed that it was indeed caused by activin. Thus, activin/EDF exerts its effect on hematopoietic cells not only on erythroid differentiation but also on at least a part of myeloid cell differentiation.
研究发现，用激活素/红细胞分化因子（EDF）处理人早幼粒细胞系HL-60后，其可分化为单核细胞/巨噬细胞样细胞。这不仅通过形态学得到证实，还表现为髓过氧化物酶颗粒的丧失和非特异性酯酶的出现。激活素结合蛋白卵泡抑素对分化的剂量依赖性抑制证实，这确实是由激活素引起的。因此，激活素/红细胞分化因子不仅对造血细胞的红系分化有作用，而且对至少一部分髓系细胞分化也有作用。
