RUDZINSKA M A
J Biophys Biochem Cytol. 1955 Sep 25;1(5):472-5. doi: 10.1083/jcb.1.5.472.
OsO(4) has many advantages over Carnoy's fixative mixture for the Feulgen nuclear staining in the protozoan Tokophrya infusionum. While Carnoy's fluid used prior to the Feulgen reaction produces shrinkage of the macronucleus and coarse clumping of its chromatin bodies, OsO(4) preserves faithfully the size and shape of the macronucleus and its chromatin material. This finding seems to be of special importance in view of the fact that electron microscopy relies on OsO(4) fixation. The satisfactory preservation of structured detail in Feulgen-stained preparations is of importance for the correlation of histochemical and morphological information.
在对原生动物点滴钟形虫进行福尔根核染色时，与卡诺固定剂混合物相比，四氧化锇（OsO₄）具有许多优势。在福尔根反应之前使用卡诺氏液会导致大核收缩及其染色质体出现粗大凝集，而四氧化锇能如实地保存大核及其染色质物质的大小和形状。鉴于电子显微镜依赖四氧化锇固定这一事实，这一发现似乎具有特别重要的意义。福尔根染色制剂中结构细节的良好保存对于组织化学和形态学信息的关联非常重要。
