Giroud F, Montmasson M P
Image Processing and Quantitative Microscopy Laboratory, Joseph Fourier University, Grenoble, France.
Anal Quant Cytol Histol. 1989 Apr;11(2):87-95.
The Feulgen reaction is used for cytophotometric quantification of nuclear DNA and texture studies of chromatin structure. It appears that fixative agents are responsible for the microscopic appearance of chromatin. In this investigation, different fixative agents mixed in various proportions were tested for their performance in automated quantitative cytology. It was determined that three factors have to be considered in the choice of a fixative: stain intensity, nuclear area and chromatin texture. In this respect, the Regaud fixative appears to be the best for automatic analysis of Feulgen-stained nuclei.
福尔根反应用于细胞核DNA的细胞光度法定量分析以及染色质结构的纹理研究。似乎固定剂决定了染色质的微观外观。在本研究中，对以不同比例混合的不同固定剂在自动定量细胞学中的性能进行了测试。结果确定，在选择固定剂时必须考虑三个因素：染色强度、核面积和染色质纹理。在这方面，雷高固定剂似乎是对福尔根染色细胞核进行自动分析的最佳选择。
