Qavi H B, Green M T, SeGall G K, Lewis D E, Hollinger F B
Division of Molecular Virology, Baylor College of Medicine, Houston, TX.
Curr Eye Res. 1992 Apr;11(4):315-23. doi: 10.3109/02713689209001785.
In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity by in situ hybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure.
在本研究中，对来自35对无症状HIV-1携带者的角膜以及10对艾滋病患者的角膜进行了分析，以检测HIV-1和HHV-6的存在情况。对这些组织进行了病毒抗原、转录本、DNA序列以及完整和感染性病毒的评估。来自两名无症状HIV-1携带者的三只角膜和来自两名艾滋病患者的三只角膜HIV-1培养呈阳性。一名无症状HIV-1携带者的三只HIV-1阳性角膜中的一只HHV-6培养也呈阳性。来自无症状HIV-1携带者的两只组织培养阳性角膜和来自艾滋病患者的两只角膜经原位杂交检测HIV-1转录活性也呈阳性。表示转录活性的标记仅限于基质角膜细胞。最为重要的是，我们能够在其中一只HIV-1培养阳性角膜的切片和培养的外周血单核细胞（PBMC）中证实HIV-1颗粒的存在。来自同一角膜的PBMC也含有疱疹病毒颗粒。本报告强化了我们早期的发现，即HIV-1和HHV-6可侵袭角膜组织，这强调了对角膜供体进行严格筛查的重要性，特别是有HIV-1暴露史的供体。
