Miquel M C, Doucet E, Riad M, Adrien J, Vergé D, Hamon M
INSERM U288, Faculté de Médecine Pitié-Salpêtrière, France.
Brain Res Mol Brain Res. 1992 Aug;14(4):357-62. doi: 10.1016/0169-328x(92)90104-j.
The effects of the selective lesion of serotoninergic neurons by an intra-raphe administration of 5,7-dihydroxytryptamine on the 5-HT1A receptor protein and the 5-HT1A receptor mRNA were examined in various regions of the rat brain using specific antibodies and an antisense riboprobe, respectively. Twenty one days after the treatment, the 5-HT1A receptor protein was no longer detected within the dorsal raphe nucleus but was still present in the hippocampus and entorhinal cortex. Quantitative in situ hybridization showed an 85% decrease in the levels of 5-HT1A receptor mRNA within the dorsal raphe nucleus, but no significant change in the hippocampus, interpeduncular nucleus and entorhinal cortex of 5,7-dihydroxytryptamine-treated rats. These data demonstrate that 5-HT1A receptors are synthesized by serotoninergic neurons in the dorsal raphe nucleus, and by neurons located postsynaptically with regard to serotoninergic projections in other areas. The unchanged levels of 5-HT1A receptor mRNA in the hippocampus, interpeduncular nucleus and entorhinal cortex three weeks after the extensive lesion of serotoninergic neurons are consistent with the absence of 5-HT1A receptor up regulation already reported under this condition.
通过向中缝内注射5,7 - 二羟基色胺对血清素能神经元进行选择性损伤，分别使用特异性抗体和反义核糖探针，在大鼠脑的各个区域检测其对5 - HT1A受体蛋白和5 - HT1A受体mRNA的影响。治疗21天后，在中缝背核内未再检测到5 - HT1A受体蛋白，但在海马体和内嗅皮质中仍存在。定量原位杂交显示，5,7 - 二羟基色胺处理的大鼠中缝背核内5 - HT1A受体mRNA水平下降了85%，但在海马体、脚间核和内嗅皮质中无显著变化。这些数据表明，5 - HT1A受体由中缝背核中的血清素能神经元以及其他区域中血清素能投射的突触后神经元合成。血清素能神经元广泛损伤三周后，海马体、脚间核和内嗅皮质中5 - HT1A受体mRNA水平未变，这与在此条件下已报道的5 - HT1A受体未上调一致。
