Maruyama I N, Brenner S
MRC Molecular Genetics Unit, Cambridge, UK.
Gene. 1992 Oct 21;120(2):135-41. doi: 10.1016/0378-1119(92)90086-5.
A bacteriophage lambda cloning vehicle has been constructed for the generation of cDNA libraries. The vector has the following properties. (1) It has a unique BamHI site engineered into the lambda gam gene. Segments of DNA can be cloned into this site and clones with an insert can be selected by their ability to grow on an Escherichia coli host lysogenic for phage P2 (Spi- phenotype). (2) When the recombinant phage infects a Cre-producing E. coli strain, a site-specific recombination event results in the excision of a plasmid replicon with the cloned insert. (3) Single-stranded DNAs can be recovered by growing helper M13 phages on bacteria harboring such plasmids. The vector, lambda MGU2, has been used to construct a nematode (Caenorhabditis elegans) cDNA library.
已构建了一种用于生成cDNA文库的λ噬菌体克隆载体。该载体具有以下特性。（1）它在λ噬菌体gam基因中设计了一个独特的BamHI位点。DNA片段可克隆到该位点，带有插入片段的克隆可通过其在对噬菌体P2溶原的大肠杆菌宿主上生长的能力来选择（Spi-表型）。（2）当重组噬菌体感染产生Cre的大肠杆菌菌株时，位点特异性重组事件导致带有克隆插入片段切除的质粒复制子。（3）单链DNA可通过在携带此类质粒的细菌上培养辅助M13噬菌体来回收。载体λMGU2已用于构建线虫（秀丽隐杆线虫）cDNA文库。
