Mizusawa S, Ward D F
Gene. 1982 Dec;20(3):317-22. doi: 10.1016/0378-1119(82)90200-1.
A phage lambda cloning vector has been constructed which contains a single site for the restriction endonuclease BamHI. Since Sau3A and Bg/II produce the same cohesive ends as BamHI, this vector can also be used to clone DNA fragments generated with either of these enzymes. We have used this vector to construct an Escherichia coli library using partial digestion with Sau3A. This vector will be most useful for applications requiring genetic analysis of cloned E. coli genes.
已构建出一种λ噬菌体克隆载体，其含有单个限制性内切核酸酶BamHI位点。由于Sau3A和Bg/II产生与BamHI相同的粘性末端，该载体也可用于克隆用这两种酶中的任何一种产生的DNA片段。我们已使用该载体通过用Sau3A进行部分酶切来构建大肠杆菌文库。该载体对于需要对克隆的大肠杆菌基因进行遗传分析的应用将最为有用。
