Pueringer R J, Bahns C C, Hunninghake G W
Department of Medicine, Department of Veterans Affairs, Iowa City, Iowa.
J Appl Physiol (1985). 1992 Aug;73(2):781-6. doi: 10.1152/jappl.1992.73.2.781.
Alveolar macrophages release greater amounts of leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) after A23187 stimulation than do blood monocytes. The mechanisms for this enhanced 5-lipoxygenase activity in alveolar macrophages are unknown. In these studies, we determined whether alveolar macrophages have greater amounts of the enzyme 5-lipoxygenase than do blood monocytes. We confirmed that alveolar macrophages released greater amounts of LTB4 after A23187 stimulation than did equivalent numbers of blood monocytes. In both the presence and absence of A23187, alveolar macrophages had greater amounts of immunoreactive 5-lipoxygenase, determined by Western analysis, on a per cell and a per protein basis than did blood monocytes. The amounts of 5-lipoxygenase enzyme in the cells roughly correlated with the amounts of LTB4 released by both types of cells. These observations suggest that A23187 stimulates alveolar macrophages to release greater amounts of LTB4 and 5-HETE than blood monocytes, in part, due to the greater amounts of 5-lipoxygenase.
与血液单核细胞相比，肺泡巨噬细胞在受到A23187刺激后释放的白三烯B4（LTB4）和5-羟基二十碳四烯酸（5-HETE）量更多。肺泡巨噬细胞中这种增强的5-脂氧合酶活性的机制尚不清楚。在这些研究中，我们确定肺泡巨噬细胞中的5-脂氧合酶含量是否比血液单核细胞更多。我们证实，与等量的血液单核细胞相比，肺泡巨噬细胞在受到A23187刺激后释放的LTB4量更多。通过蛋白质免疫印迹分析确定，无论有无A23187刺激，肺泡巨噬细胞中免疫反应性5-脂氧合酶的含量在每细胞和每蛋白基础上均高于血液单核细胞。细胞中5-脂氧合酶的含量与两种细胞释放的LTB4量大致相关。这些观察结果表明，A23187刺激肺泡巨噬细胞释放比血液单核细胞更多的LTB4和5-HETE，部分原因是5-脂氧合酶含量更高。
