外周血单个核细胞中丙型肝炎病毒RNA复制形式的检测

Detection of replicative form of hepatitis C virus RNA in peripheral blood mononuclear cells.

作者信息

Wang J T, Sheu J C, Lin J T, Wang T H, Chen D S

机构信息

Department of Internal Medicine, National Taiwan University Hospital, Taipei, Republic of China.

出版信息

J Infect Dis. 1992 Nov;166(5):1167-9. doi: 10.1093/infdis/166.5.1167.

DOI:10.1093/infdis/166.5.1167

PMID:1328405

Abstract

Paired samples of plasma and peripheral blood mononuclear cells (PBMC) from 7 patients with posttransfusion hepatitis C were collected and studied for the presence of replicative forms of hepatitis C virus (HCV). RNA was extracted and tested for both positive and negative strands of HCV RNA by polymerase chain reaction. Positive-strand RNA of HCV was found in both plasma and PBMC of all 7 patients. However, negative-strand RNA was found in PBMC of 3 patients while none was found in the plasma. Levels of negative-strand RNA were about 10-100 times less than those of positive-strand RNA by semiquantification with serial dilution of viral cDNA. The results suggest that active infection and replication of HCV in PBMC is present in patients with posttransfusion hepatitis C and implicate the extrahepatic infection of HCV.

摘要

收集了7例输血后丙型肝炎患者的配对血浆和外周血单个核细胞（PBMC）样本，研究丙型肝炎病毒（HCV）复制形式的存在情况。提取RNA，通过聚合酶链反应检测HCV RNA的正链和负链。在所有7例患者的血浆和PBMC中均发现了HCV正链RNA。然而，在3例患者的PBMC中发现了负链RNA，而血浆中未发现。通过对病毒cDNA进行系列稀释半定量分析，负链RNA水平比正链RNA低约10 - 100倍。结果表明，输血后丙型肝炎患者的PBMC中存在HCV的活跃感染和复制，提示HCV存在肝外感染。