Sunil-Chandra N P, Efstathiou S, Arno J, Nash A A
Department of Pathology, University of Cambridge, U.K.
J Gen Virol. 1992 Sep;73 ( Pt 9):2347-56. doi: 10.1099/0022-1317-73-9-2347.
The primary infection of BALB/c mice with murine herpesvirus 68 (MHV-68) was investigated. When the virus was introduced intranasally, the lung was the main tissue infected, the virus being associated with alveolar epithelium and mononuclear cells. A productive infection lasted for 10 days, after which viral DNA could be detected by in situ hybridization up to 30 days after infection. At that time lymphoproliferative accumulations were also observed in the lung, with formation of germinal centres. Virus could also be recovered from the heart, kidney, adrenal gland and spleen during the primary infection. In addition, the spleen appeared to be the major site of virus persistence, with latently infected cells detected up to 90 days post-infection. During the primary infection, there was atrophy of the thymus and spleen of clinically sick animals. In contrast, lymphoproliferative responses, typified by splenomegaly, were frequently seen in asymptomatic animals. The pattern of infection observed in MHV-68-infected mice is similar to that seen in infectious mononucleosis of man following Epstein-Barr virus infection. The model described in this paper may prove to be useful in studying natural gamma-herpesvirus infections of man and domestic animals.
对BALB/c小鼠感染鼠疱疹病毒68（MHV-68）的原发性感染进行了研究。经鼻接种该病毒后，肺是主要的感染组织，病毒与肺泡上皮细胞和单核细胞相关。活跃感染持续10天，此后在感染后30天内均可通过原位杂交检测到病毒DNA。此时在肺中也观察到淋巴细胞增殖性聚集，并形成生发中心。在原发性感染期间，病毒也可从心脏、肾脏、肾上腺和脾脏中分离出来。此外，脾脏似乎是病毒持续存在的主要部位，在感染后90天内均可检测到潜伏感染的细胞。在原发性感染期间，临床患病动物的胸腺和脾脏出现萎缩。相比之下，无症状动物中经常可见以脾肿大为特征的淋巴细胞增殖反应。在感染MHV-68的小鼠中观察到的感染模式与人类感染爱泼斯坦-巴尔病毒后传染性单核细胞增多症的模式相似。本文所述模型可能被证明对研究人类和家畜的自然γ-疱疹病毒感染有用。
