Ehtisham S, Sunil-Chandra N P, Nash A A
Department of Pathology, University of Cambridge, United Kingdom.
J Virol. 1993 Sep;67(9):5247-52. doi: 10.1128/JVI.67.9.5247-5252.1993.
Murine gammaherpesvirus is a natural pathogen of wild mice. The virus infects alveolar cells and spleen cells during the primary infection and establishes a latent/persistent infection in B lymphocytes. Little is known about the immunological response to gammaherpesviruses during a primary infection. To address this issue, we investigated the pathogenesis of murine herpesvirus 68 (MHV-68) infection in mice deficient in CD4 or CD8 T-cell populations. Infection of the lung and spleen were greatly exacerbated in CD8-deficient mice, reflected by elevated virus titers in the lung and an increase in the number of infected splenocytes located around germinal centers. This finding contrasts with clearance of virus from the lung and spleen by day 12 postinfection in CD4-depleted animals. These data clearly indicate a major role for CD8 T cells in recovery from an acute MHV-68 infection. Whereas CD4 T cells fail to influence the course of infection in the lung, they do contribute to lymphoproliferation seen in the spleen (splenomegaly) during the primary infection. The significance of these results are discussed in relation to the immune response to other herpesviruses, in particular Epstein-Barr virus, with which MHV-68 shares similar molecular and biological properties.
鼠γ疱疹病毒是野生小鼠的一种天然病原体。该病毒在初次感染期间感染肺泡细胞和脾细胞，并在B淋巴细胞中建立潜伏/持续感染。关于初次感染期间对γ疱疹病毒的免疫反应知之甚少。为了解决这个问题，我们研究了在缺乏CD4或CD8 T细胞群体的小鼠中鼠疱疹病毒68（MHV - 68）感染的发病机制。在CD8缺陷小鼠中，肺和脾的感染大大加剧，表现为肺中病毒滴度升高以及生发中心周围感染脾细胞数量增加。这一发现与CD4耗尽动物在感染后第12天从肺和脾中清除病毒形成对比。这些数据清楚地表明CD8 T细胞在急性MHV - 68感染恢复中起主要作用。虽然CD4 T细胞不影响肺部感染进程，但它们确实在初次感染期间对脾中出现的淋巴细胞增殖（脾肿大）有作用。这些结果的意义将结合对其他疱疹病毒，特别是与MHV - 68具有相似分子和生物学特性的爱泼斯坦 - 巴尔病毒的免疫反应进行讨论。
