Renvert S, Wikström M, Helmersson M, Dahlén G, Claffey N
School of Dental Hygiene, Public Dental Service, Kristianstad, Sweden.
J Periodontol. 1992 Oct;63(10):797-801. doi: 10.1902/jop.1992.63.10.797.
The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Subgingival samples using both methods were taken from three surfaces in each of 12 patients at the following time points: at each of two appointments one week apart before treatment and at each of two appointments 12 and 13 weeks following treatment. Microbiological analyses were undertaken to determine the total number of colony forming units, the proportions of suspected periodontal pathogens, and the number of spirochetes using phase contrast microscopy. Significantly higher numbers of colony forming units and spirochetes were found for paper point sampling both before and after treatment.
龈下菌斑中特定细菌的存在已被用作活动性牙周病的指标。龈下采样技术可能会推测性地影响所报告微生物的鉴定和计数。在本研究中，对纸尖采样和龈下刮治器采样进行了比较。使用这两种方法从12名患者的每个患者的三个表面在以下时间点采集龈下样本：治疗前相隔一周的两次就诊时，以及治疗后12周和13周的两次就诊时。进行微生物学分析以确定菌落形成单位的总数、疑似牙周病原体的比例以及使用相差显微镜观察的螺旋体数量。在治疗前后，纸尖采样均发现菌落形成单位和螺旋体的数量明显更高。
