细菌胞外蛋白酶生物合成的研究。I. 粘质沙雷氏菌、合成培养基和明胶培养基
Studies on the biosynthesis of extracellular proteases by bacteria. I. Serratia marcescens, synthetic and gelatin media.
作者信息
CASTANEDA-AGULLO M
出版信息
J Gen Physiol. 1956 Jan 20;39(3):369-75. doi: 10.1085/jgp.39.3.369.
Abstract
Apparently the extracellular protease of S. marcescens is present in protein- or protein derivative-free media only when the conditions favor an abundant growth and bacterial conglomerates are formed, which possibly could be explained by assuming that the proteins of dead bacteria or proteic metabolic products act as inducers of protease formation. Dilute solutions of gelatin induce the production of proteolytic activity after a short period of time and its rapid increase before the initial number of bacteria has been duplicated. Calcium is necessary to preserve the activity of the enzyme formed in synthetic media but not that produced in gelatin.
摘要
显然，只有在有利于大量生长并形成细菌聚集体的条件下，粘质沙雷氏菌的细胞外蛋白酶才会出现在无蛋白质或无蛋白质衍生物的培养基中，这可能可以通过假设死细菌的蛋白质或蛋白质代谢产物作为蛋白酶形成的诱导剂来解释。明胶稀溶液在短时间后会诱导蛋白水解活性的产生，并且在细菌初始数量翻倍之前其活性会迅速增加。钙对于维持在合成培养基中形成的酶的活性是必需的，但对于在明胶中产生的酶则不是必需的。
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本文引用的文献
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Further studies of the effect of the medium on the production of bacterial gelatinase.关于培养基对细菌明胶酶产生影响的进一步研究。
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The formation of bacterial proteases, especially in synthetic media.细菌蛋白酶的形成，尤其是在合成培养基中的形成。
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[The role of calcium in the activity and stability of various bacterial proteinases].[钙在各种细菌蛋白酶活性和稳定性中的作用]
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[Physiological factors determining the presence of proteinase in the cultures of Micrococcus lysodeikticus].[决定溶壁微球菌培养物中蛋白酶存在的生理因素]
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