CASTANEDA-AGULLO M
J Gen Physiol. 1956 Jan 20;39(3):369-75. doi: 10.1085/jgp.39.3.369.
Apparently the extracellular protease of S. marcescens is present in protein- or protein derivative-free media only when the conditions favor an abundant growth and bacterial conglomerates are formed, which possibly could be explained by assuming that the proteins of dead bacteria or proteic metabolic products act as inducers of protease formation. Dilute solutions of gelatin induce the production of proteolytic activity after a short period of time and its rapid increase before the initial number of bacteria has been duplicated. Calcium is necessary to preserve the activity of the enzyme formed in synthetic media but not that produced in gelatin.
显然,只有在有利于大量生长并形成细菌聚集体的条件下,粘质沙雷氏菌的细胞外蛋白酶才会出现在无蛋白质或无蛋白质衍生物的培养基中,这可能可以通过假设死细菌的蛋白质或蛋白质代谢产物作为蛋白酶形成的诱导剂来解释。明胶稀溶液在短时间后会诱导蛋白水解活性的产生,并且在细菌初始数量翻倍之前其活性会迅速增加。钙对于维持在合成培养基中形成的酶的活性是必需的,但对于在明胶中产生的酶则不是必需的。