Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Unidad Profesional Adolfo López Mateos, Mexico City 07738, Mexico.
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, Mexico City 11340, Mexico.
Int J Mol Sci. 2024 Aug 30;25(17):9430. doi: 10.3390/ijms25179430.
Riboflavin, an essential vitamin for humans, is extensively used in various industries, with its global demand being met through fermentative processes. is a novel dimorphic yeast species capable of producing riboflavin. However, the nutritional factors affecting riboflavin production in this yeast species remain unknown. Therefore, we conducted a kinetic study on the effects of various nutritional factors-carbon and energy sources, nitrogen sources, vitamins, and amino acids-on batch riboflavin production by . Batch experiments were performed in a bubble column bioreactor to evaluate cell growth, substrate consumption, and riboflavin production. The highest riboflavin production was obtained when the yeast growth medium was supplemented with glucose, ammonium sulfate, biotin, and glycine. Using these chemical components, along with the mineral salts from Castañeda-Agullo's culture medium, we formulated a novel, low-cost, and effective culture medium (the RGE medium) for riboflavin production by . This medium resulted in the highest levels of riboflavin production and volumetric productivity, reaching 16.68 mg/L and 0.713 mg/L·h, respectively, within 21 h of incubation. These findings suggest that , with its shorter incubation time, could improve the efficiency and cost-effectiveness of industrial riboflavin production, paving the way for more sustainable production methods.
核黄素是人体必需的维生素，广泛应用于各个行业，其全球需求通过发酵工艺得到满足。是一种新型的二态酵母物种，能够生产核黄素。然而，这种酵母生产核黄素的营养因素尚不清楚。因此，我们对各种营养因素（碳源和能源、氮源、维生素和氨基酸）对分批发酵生产核黄素的影响进行了动力学研究。在鼓泡塔式生物反应器中进行分批实验，以评估细胞生长、基质消耗和核黄素生产。当酵母生长培养基中添加葡萄糖、硫酸铵、生物素和甘氨酸时，核黄素的产量最高。使用这些化学成分以及 Castañeda-Agullo 培养基中的无机盐，我们为 设计了一种新型的、低成本的、有效的核黄素生产培养基（RGE 培养基）。该培养基在 21 小时的孵育时间内，达到了 16.68mg/L 和 0.713mg/L·h 的最高核黄素产量和比生产率。这些发现表明， 具有更短的孵育时间，可以提高工业核黄素生产的效率和成本效益，为更可持续的生产方法铺平道路。
