人类巨细胞病毒的UL16基因编码一种糖蛋白，该糖蛋白在体外生长中并非必需。
The UL16 gene of human cytomegalovirus encodes a glycoprotein that is dispensable for growth in vitro.
作者信息
Kaye J, Browne H, Stoffel M, Minson T
机构信息
Department of Pathology, University of Cambridge, United Kingdom.
出版信息
J Virol. 1992 Nov;66(11):6609-15. doi: 10.1128/JVI.66.11.6609-6615.1992.
Abstract
The UL16 gene of human cytomegalovirus (HCMV) encodes a predicted translation product with features characteristic of glycoproteins (signal and anchor sequences and eight potential N-linked glycosylation sites). Antisera were raised against the UL16 gene product expressed in Escherichia coli as a beta-galactosidase fusion protein. The antisera detected a 50-kDa glycoprotein in HCMV-infected cells that was absent from purified virions. The UL16 glycoprotein was synthesized at early times after infection and accumulated to the highest levels at late times after infection. A recombinant HCMV in which UL16 coding sequences were interrupted by a lacZ expression cassette was constructed by insertional mutagenesis. Analysis of the phenotype of the recombinant virus indicated that the UL16 gene product is nonessential for virus infectivity and growth in tissue culture.
摘要
人巨细胞病毒（HCMV）的UL16基因编码一种预测的翻译产物，具有糖蛋白的特征（信号和锚定序列以及八个潜在的N-连接糖基化位点）。制备了针对在大肠杆菌中作为β-半乳糖苷酶融合蛋白表达的UL16基因产物的抗血清。该抗血清在HCMV感染的细胞中检测到一种50 kDa的糖蛋白，而在纯化的病毒粒子中不存在。UL16糖蛋白在感染后的早期合成，并在感染后的晚期积累到最高水平。通过插入诱变构建了一种重组HCMV，其中UL16编码序列被lacZ表达盒中断。对重组病毒表型的分析表明，UL16基因产物对于病毒在组织培养中的感染性和生长不是必需的。
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