Chou S, Marousek G I
Medical Service, VA Medical Center, Portland, Oregon.
Virology. 1992 Nov;191(1):523-8. doi: 10.1016/0042-6822(92)90224-d.
The envelope glycoprotein B (gB) coding sequences of two strains of human herpesvirus-6 (HHV6 GS and Z29) were determined by sequencing a 2.5-kb open reading frame adjacent to the DNA polymerase sequence. The deduced primary translation product is 830 amino acids in length and is 96% conserved between the two divergent strains with no localized hypervariability noted. It contains the expected signal and transmembrane sequence motifs as well as a putative site of protease cleavage. There was 39% amino acid identity with the gB of human cytomegalovirus (CMV, strain AD169). HHV6-CMV gB peptide homology was evident through the entire sequence, but was especially strong in the amino-terminal portion of CMV gp55, which contains linear and conformational epitopes recognized by CMV-neutralizing antibodies. All 10 cysteine residues of HHV6 gB match corresponding residues of CMV gB. Sequence data suggest strong structural similarity and possible immunologic cross-reactivity of gB from the two viruses.
通过对与DNA聚合酶序列相邻的一个2.5 kb开放阅读框进行测序，确定了两株人疱疹病毒6型（HHV6 GS和Z29）的包膜糖蛋白B（gB）编码序列。推导的初级翻译产物长度为830个氨基酸，在这两个不同的毒株之间有96%的保守性，未发现局部高变区。它包含预期的信号和跨膜序列基序以及一个假定的蛋白酶切割位点。与人类巨细胞病毒（CMV，AD169株）的gB有39%的氨基酸同一性。HHV6-CMV gB肽的同源性在整个序列中都很明显，但在CMV gp55的氨基末端部分尤其强，该部分包含被CMV中和抗体识别的线性和构象表位。HHV6 gB的所有10个半胱氨酸残基与CMV gB的相应残基匹配。序列数据表明这两种病毒的gB具有很强的结构相似性和可能的免疫交叉反应性。
