LEON M A
J Exp Med. 1956 Mar 1;103(3):285-93. doi: 10.1084/jem.103.3.285.
Quantitative methods have been developed for the estimation of C'3 and properdin. With these methods, the kinetics of the over-all reaction between properdin, zymosan, and C'3 were investigated. The reaction followed first order kinetics, provided zymosan was in excess, and the serum not diluted beyond 1/4. The reaction rate was proportional to the temperature, and an activation energy of 20,500 calories/mole was found. The distinction between total properdin and available serum properdin is discussed.
已经开发出定量方法来估算补体C3和备解素。运用这些方法,对备解素、酵母聚糖和补体C3之间的整体反应动力学进行了研究。如果酵母聚糖过量且血清稀释不超过1/4,则该反应遵循一级动力学。反应速率与温度成正比,并且发现其活化能为20,500卡/摩尔。文中还讨论了总备解素和血清中可利用备解素之间的区别。