de la Fuente R, Awan A R, Field H J
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Antiviral Res. 1992 May;18(1):77-89. doi: 10.1016/0166-3542(92)90007-r.
Equine herpesvirus type 1 (EHV-1) was sensitive to the nucleoside analogue penciclovir (PCV) when tested in tissue culture; the ED50 was 1.6 micrograms/ml. Drug-resistant mutants were selected which were found to be TK-defective and approx. 45-fold less sensitive to PCV compared with the parental strain. PCV was compared with the phosphonyl derivative, HPMPA in mice infected with EHV-1. Both drugs were shown to be effective in vivo, limiting wild-type virus replication in respiratory tissues, and reducing viraemia. The treated mice also showed less clinical signs and reduced histopathology compared with placebo-treated controls. The establishment of latent EHV-1 in the mice, however, was not prevented. The results obtained with mice suggest that antiviral chemotherapy may be practical in the horse and that this possibility is worthy of further investigation in the natural host.
在组织培养中进行测试时，1型马疱疹病毒（EHV-1）对核苷类似物喷昔洛韦（PCV）敏感；半数有效剂量为1.6微克/毫升。筛选出了耐药突变体，发现其胸苷激酶（TK）缺陷，与亲本菌株相比，对PCV的敏感性约低45倍。在感染EHV-1的小鼠中，将PCV与膦酰衍生物HPMPA进行了比较。两种药物在体内均显示有效，可限制呼吸道组织中的野生型病毒复制，并减少病毒血症。与安慰剂处理的对照组相比，接受治疗的小鼠临床症状也更少，组织病理学变化也减轻。然而，并未阻止EHV-1在小鼠中建立潜伏感染。在小鼠中获得的结果表明，抗病毒化疗在马匹中可能可行，这种可能性值得在自然宿主中进一步研究。
