补体、血小板活化因子和白三烯B4在反向被动Arthus反应中的作用。

The role of complement, platelet-activating factor and leukotriene B4 in a reversed passive Arthus reaction.

Rossi A G, Norman K E, Donigi-Gale D, Shoupe T S, Edwards R, Williams T J

Department of Applied Pharmacology, National Heart & Lung Institute, London.

Br J Pharmacol. 1992 Sep;107(1):44-9. doi: 10.1111/j.1476-5381.1992.tb14461.x.

Abstract

The mechanisms underlying oedema formation induced in a reversed passive Arthus (RPA) reaction and, for comparison, in response to zymosan in rabbit skin were investigated. 2. Oedema formation at skin sites was quantified by the accumulation of intravenously-injected 125I-labelled human serum albumin. 3. Recombinant soluble complement receptor type 1 (sCR1), administered locally in rabbit skin, suppressed oedema formation induced in the RPA reaction and by zymosan. 4. The platelet-activating factor (PAF) antagonists, WEB 2086 and PF10040 administered locally, inhibited oedema formation induced in the RPA reaction and by PAF but not by zymosan. 5. A locally administered leukotriene B4 (LTB4) antagonist, LY-255283, inhibited oedema formation induced by LTB4 but did not inhibit oedema responses to PAF, zymosan or the RPA reaction. 6. The results demonstrate a role for complement in oedema formation in both the RPA reaction and in response to zymosan. An important contribution by PAF is indicated in the RPA reaction but not in response to zymosan whereas no evidence was obtained to suggest a role for LTB4 in either inflammatory response.

摘要

研究了在兔皮肤中反向被动Arthus（RPA）反应以及作为对照对酵母聚糖产生反应时引起水肿形成的机制。2. 通过静脉注射125I标记的人血清白蛋白的蓄积来定量皮肤部位的水肿形成。3. 在兔皮肤局部给予重组可溶性补体受体1型（sCR1），可抑制RPA反应和酵母聚糖诱导的水肿形成。4. 局部给予血小板活化因子（PAF）拮抗剂WEB 2086和PF10040，可抑制RPA反应和PAF诱导的水肿形成，但不能抑制酵母聚糖诱导的水肿形成。5. 局部给予白三烯B4（LTB4）拮抗剂LY-255283，可抑制LTB4诱导的水肿形成，但不能抑制对PAF、酵母聚糖或RPA反应的水肿反应。6. 结果表明补体在RPA反应和对酵母聚糖反应的水肿形成中均起作用。PAF在RPA反应中有重要作用，但在对酵母聚糖的反应中不起作用，而未获得证据表明LTB4在任何一种炎症反应中起作用。