Schmidt M, Friedrich T, Wallrath J, Ohnishi T, Weiss H
Institut für Biochemie, Universität Düsseldorf, Germany.
FEBS Lett. 1992 Nov 16;313(1):8-11. doi: 10.1016/0014-5793(92)81172-i.
The NADH:ubiquinone oxidoreductase (complex I) of mitochondria is constructed from two arms arranged perpendicular to each other. The peripheral arm protruding into the matrix contains the proximal section of the electron pathway, and the membrane arm with all mitochondrially encoded subunits contains the distal section of the electron pathway. When Neurospora crassa is grown under manganese limitation the formation of the peripheral arm is disturbed, but the membrane arm containing the iron-sulfur cluster N-2, is accumulated. An extra-polypeptide, assumed to be a chaperone, is found to be associated with this pre-assembled membrane arm.
线粒体的NADH：泛醌氧化还原酶（复合体I）由两条相互垂直排列的臂构成。伸入基质的外周臂包含电子传递途径的近端部分，而包含所有线粒体编码亚基的膜臂则包含电子传递途径的远端部分。当粗糙脉孢菌在锰限制条件下生长时，外周臂的形成受到干扰，但含有铁硫簇N-2的膜臂会积累。发现一种额外的多肽（假定为伴侣蛋白）与这种预先组装好的膜臂相关联。
