Finel M
Department of Medical Chemistry, University of Helsinki, Finland.
J Bioenerg Biomembr. 1993 Aug;25(4):357-66. doi: 10.1007/BF00762461.
The proton-translocating NADH:ubiquinone oxidoreductase (complex I) is a large, multi-subunit and multi-redox centre enzyme which is found in the mitochondrial inner membrane and plasma membrane of some bacteria. In this minireview an attempt has been made to critically discuss selected topics in the structure and function of this enzyme. A special emphasis is given to the iron-sulphur cluster and to the proteins that may bind them. Previous suggestions for the mechanism of proton-translocation by complex I are discussed. Subcomplexes that contain several but not all of the subunits of the intact mitochondrial enzyme are described and analysed in order to identify the functional core of the enzyme. The data on the trans-membrane organisation of several subunits is examined. It is hoped that most of the suggestions as well as the questions raised here could be experimentally tested in the near future.
质子转运型NADH：泛醌氧化还原酶（复合体I）是一种大型的多亚基、多氧化还原中心酶，存在于线粒体内膜和某些细菌的质膜中。在这篇小型综述中，我们试图批判性地讨论该酶结构和功能方面的一些选定主题。特别强调了铁硫簇以及可能结合它们的蛋白质。讨论了之前关于复合体I质子转运机制的建议。描述并分析了包含完整线粒体酶的几个但并非全部亚基的亚复合体，以确定该酶的功能核心。研究了几个亚基的跨膜组织数据。希望这里提出的大多数建议和问题在不久的将来能够通过实验进行验证。
