Vostrov A A, Malinin A Iu, Rybchin V N, Sravchevskiĭ A N
Genetika. 1992 Jul;28(7):186-8.
Prophage of the temperate coliphage N15 is a linear plasmid with covalently closed ends. The central part of plasmid N15 genome responsible for vegetative phage growth was replaced by DNA fragments containing genes for selective markers which have unique restriction sites. As a result a family of linear plasmid vectors was constructed. Their size is about 20 kb and their capacity is comparable with that of cosmid vectors.
温和型大肠杆菌噬菌体N15的原噬菌体是一种具有共价闭合末端的线性质粒。质粒N15基因组中负责营养型噬菌体生长的中央部分被含有带有独特限制酶切位点的选择标记基因的DNA片段所取代。结果构建了一系列线性质粒载体。它们的大小约为20 kb,其容量与黏粒载体相当。