Gaytan F, Aceitero J, Lucena C, Aguilar E, Pinilla L, Garnelo P, Bellido C
Biology Section, School of Medicine, University of Córdoba, Spain.
J Androl. 1992 Sep-Oct;13(5):387-97.
The proliferation and differentiation of mast cells and Leydig cells were studied in adult sham operated or hypophysectomized rats after the administration of ethylene dimethane sulphonate (EDS) and in prepubertal rats after neonatal treatment with a gonadotropin-releasing hormone (GnRH) antagonist (Organon 30276; Oss, The Netherlands). After treatment with EDS, two proliferative waves were found. On day 3, several interstitial cell types proliferated, whereas mitotic cells corresponded to differentiating Leydig cells and mast cells around day 20. Differentiating Leydig cells showed a higher mitotic index than that of differentiating mast cells. Hypophysectomized animals showed high mitotic activity 3 days after treatment, but 21 days after treatment differentiating Leydig cells were absent and proliferative activity was reduced. The number of mast cells increased from day 15 to day 30 in EDS-treated rats and from day 15 to day 50 in hypophysectomized, EDS-treated rats. GnRH antagonist-treated rats showed poorly differentiated Leydig cells and abundant mitotic figures on day 23. Proliferation and differentiation of Leydig cells occurred concomitantly with the proliferation and differentiation of mast cells between 23 and 30 days of age. These results suggest that Leydig cells and mast cells in the rat testis share some common regulatory factors.
在成年假手术或垂体切除的大鼠中，于给予乙烷二甲磺酸盐（EDS）后，以及在新生期用促性腺激素释放激素（GnRH）拮抗剂（荷兰欧加农公司生产的Organon 30276）处理的青春期前大鼠中，研究了肥大细胞和睾丸间质细胞的增殖与分化。用EDS处理后，发现有两个增殖波。在第3天，几种间质细胞类型增殖，而有丝分裂细胞在第20天左右对应于分化中的睾丸间质细胞和肥大细胞。分化中的睾丸间质细胞的有丝分裂指数高于分化中的肥大细胞。垂体切除的动物在处理后3天显示出高有丝分裂活性，但在处理后21天，分化中的睾丸间质细胞缺失且增殖活性降低。在EDS处理的大鼠中，肥大细胞数量从第15天增加到第30天，在垂体切除且经EDS处理的大鼠中，从第15天增加到第50天。用GnRH拮抗剂处理的大鼠在第23天显示出分化不良的睾丸间质细胞和丰富的有丝分裂图像。在23至30日龄之间，睾丸间质细胞的增殖和分化与肥大细胞的增殖和分化同时发生。这些结果表明，大鼠睾丸中的睾丸间质细胞和肥大细胞共享一些共同的调节因子。
