Imanishi O, Fujisawa M, Matsumoto O, Kamidono S
Department of Urology, Kobe University School of Medicine, Japan.
J Reprod Fertil. 1992 Sep;96(1):355-61. doi: 10.1530/jrf.0.0960355.
In the epididymis of young rats, activities of DNA polymerases alpha, beta and gamma and DNA topoisomerase I decreased after castration. DNA polymerase alpha and gamma increased with androgen administration and activity reached 81.3% and 78.0%, respectively, of the activity in the sham-operated group on day 21. Activity of DNA polymerase beta remained at the activity of day 7 during androgen administration and was almost the same as that in the sham-operated group on day 21. DNA topoisomerase I activity showed a slight increase with androgen administration and reached 50.3% of that in the sham-operated group. The activities of these enzymes were not fully restored to those in the sham-operated group. These results indicate that in young rats activities of epididymal DNA polymerase alpha and gamma and DNA topoisomerase I are partially, and that of DNA polymerase beta wholly, dependent on androgens and may provide a means of investigating the regulation of epididymal cell proliferation.
在幼年大鼠的附睾中，去势后DNA聚合酶α、β和γ以及DNA拓扑异构酶I的活性降低。给予雄激素后，DNA聚合酶α和γ的活性增加，在第21天分别达到假手术组活性的81.3%和78.0%。在给予雄激素期间，DNA聚合酶β的活性维持在第7天的水平，在第21天与假手术组几乎相同。DNA拓扑异构酶I的活性在给予雄激素后略有增加，达到假手术组的50.3%。这些酶的活性并未完全恢复到假手术组的水平。这些结果表明，在幼年大鼠中，附睾DNA聚合酶α和γ以及DNA拓扑异构酶I的活性部分依赖雄激素，而DNA聚合酶β的活性完全依赖雄激素，这可能为研究附睾细胞增殖的调控提供一种方法。
