Okuda Y, Fujisawa M, Matsumoto O, Kamidono S
Department of Urology, Kobe University School of Medicine, Japan.
J Urol. 1991 Jan;145(1):188-91. doi: 10.1016/s0022-5347(17)38288-5.
The effects of testosterone on the activities of DNA polymerase alpha, beta, and gamma as well as topoisomerase I (all enzymes involved in DNA synthesis) were examined in the rat ventral prostate. The activities of these four enzymes decreased gradually after castration in comparison with normal controls, and continued to decrease until the 14th day. Enzyme activities were almost restored to normal within 48 to 72 hr. of the commencement of daily testosterone injections (0.3 mg./0.2 ml.), and increased successively until the 14th day. The wet weight, protein content, and DNA content of the rat ventral prostate decreased after castration, while following testosterone therapy all these parameters increased to equal or exceed the control levels. These results suggested that the activities of DNA polymerase alpha, beta, and gamma and topoisomerase I are at least partially regulated by testosterone, and that these enzymes play an important role in the regulation of prostatic cellular proliferation.
在大鼠腹侧前列腺中检测了睾酮对DNA聚合酶α、β和γ以及拓扑异构酶I（所有参与DNA合成的酶）活性的影响。与正常对照组相比，去势后这四种酶的活性逐渐降低，并持续下降直至第14天。在每日注射睾酮（0.3毫克/0.2毫升）开始后的48至72小时内，酶活性几乎恢复正常，并持续上升直至第14天。大鼠腹侧前列腺的湿重、蛋白质含量和DNA含量在去势后降低，而在睾酮治疗后，所有这些参数均增加至等于或超过对照水平。这些结果表明，DNA聚合酶α、β和γ以及拓扑异构酶I的活性至少部分受睾酮调节，并且这些酶在前列腺细胞增殖的调节中起重要作用。
