Simmons G M
Department of Biology, City College of the City University of New York, New York 10031.
Mol Biol Evol. 1992 Nov;9(6):1050-60. doi: 10.1093/oxfordjournals.molbev.a040774.
The hobo family of transposable elements, one of three transposable-element families that cause hybrid dysgenesis in Drosophila melanogaster, appears to be present in all members of the D. melanogaster species complex: D. melanogaster, D. simulans, D. mauritiana, and D. sechellia. Some hobo-hybridizing sequences are also found in the other members of the melanogaster subgroup and in many members of the related montium subgroup. Surveys of older isofemale lines of D. melanogaster suggest that complete hobo elements were absent prior to 50 years ago and that hobo has recently been introduced into the species by horizontal transfer. To test the horizontal transfer hypothesis, the 2.6-kb XhoI fragments of hobo elements from D. melanogaster, D. simulans, and D. mauritiana were cloned and sequenced. The DNA sequences reveal an extremely low level of divergence and support the conclusion that the active hobo element has been horizontally transferred into or among these species in the recent past.
转座因子的hobo家族是在黑腹果蝇中导致杂种不育的三个转座因子家族之一，似乎存在于黑腹果蝇物种复合体的所有成员中：黑腹果蝇、拟果蝇、毛里求斯果蝇和塞舌尔果蝇。在黑腹果蝇亚组的其他成员以及相关的山地果蝇亚组的许多成员中也发现了一些与hobo杂交的序列。对黑腹果蝇较老的同雌系的调查表明，完整的hobo元件在50年前并不存在，并且hobo最近通过水平转移被引入该物种。为了检验水平转移假说，克隆并测序了来自黑腹果蝇、拟果蝇和毛里求斯果蝇的hobo元件的2.6kb XhoI片段。DNA序列显示出极低的差异水平，并支持了活跃的hobo元件在最近被水平转移到这些物种中或在这些物种之间转移的结论。
