Ganushkina L A
Med Parazitol (Mosk). 1992 Mar-Apr(2):47-50.
A relationship was found between Pseudomonas suppression by phytobacteriomycin (PBM) in the mosquito larval gut and the mosquito vectorial capacity. The suppression of Pseudomonas bacteria in larvae caused a decrease in the vectorial capacity of emerged imagines. The results of the in vivo tests were verified in vitro with the culture of bacteria isolated from mosquito larva in liquid medium with pH 8.4 which is equal to intestinal pH of Ae. aegypti larva inside the peritrophic membrane. The laboratory Ae. aegypti fed on sugar with PBM addition (titer 100 U/ml) were 18.9% lees sensitive to malaria parasite. Without additional blood meal, mosquito sensitivity to malaria parasite sharply decreases at day 14, without substantially changing their intestinal microflora.
在蚊虫幼虫肠道中，植物细菌霉素（PBM）对铜绿假单胞菌的抑制作用与蚊虫的传播能力之间存在关联。幼虫体内铜绿假单胞菌的抑制导致羽化后的成虫传播能力下降。体内试验结果在体外得到验证，所用细菌是从蚊虫幼虫中分离出来的，在pH 8.4的液体培养基中培养，该pH值与埃及伊蚊幼虫围食膜内的肠道pH值相同。在实验室中，给埃及伊蚊喂食添加了PBM（效价为100 U/ml）的糖水，其对疟原虫的敏感性降低了18.9%。在没有额外血餐的情况下，蚊虫对疟原虫的敏感性在第14天急剧下降，但其肠道微生物群没有实质性变化。
