Neckers L M, Rosolen A, Whitesell L
Tumor Cell Biology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
J Immunother (1991). 1992 Oct;12(3):162-6. doi: 10.1097/00002371-199210000-00003.
To understand the role of individual genes in regulating biological processes, one must be able to interfere specifically with either their expression or function. While monoclonal antibodies have proven very useful in studying cell surface proteins, the specific inhibition of intracellular proteins in viable cells is a much more difficult problem. The goal of antisense technology is to develop small oligonucleotides, plasmids, or retroviral vectors that can be introduced easily into viable cells in order to inhibit gene products specifically. In this report, we will describe our use of antisense DNA and RNA to study the role of the NMYC proto-oncogene in neuroectodermal cell growth and differentiation.
为了解单个基因在调节生物过程中的作用，必须能够特异性地干扰其表达或功能。虽然单克隆抗体已被证明在研究细胞表面蛋白方面非常有用，但在活细胞中特异性抑制细胞内蛋白是一个困难得多的问题。反义技术的目标是开发能够轻松导入活细胞以特异性抑制基因产物的小寡核苷酸、质粒或逆转录病毒载体。在本报告中，我们将描述我们使用反义DNA和RNA来研究NMYC原癌基因在神经外胚层细胞生长和分化中的作用。
