Takahisa M, Togashi S, Ueda R, Mikuni M, Tsurumura S, Kondo K, Miyake T
Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Gene. 1992 Nov 16;121(2):343-6. doi: 10.1016/0378-1119(92)90141-b.
A P element (PE)-induced Drosophila melanogaster mutation, hari, affects the formation of the bristle mechanosensory organ in the adult fly. In this mutation, the site of PE insertion is in the first intron of the gene (CycA) encoding cyclin A (CycA). In order to analyze the hari mutant at the molecular level, we cloned and sequenced the cDNA and genomic DNA encoding CycA. CycA has seven exons and six introns, and its transcription unit spans 6 kb. All exon-intron junctions are compatible with the GT/AG consensus. Results of primer extension analysis and RNase protection assay indicate that CycA has major and minor transcription start points (tsp). To our knowledge, this is the first report on the CycA genomic sequence from a multicellular organism.
P 因子（PE）诱导的果蝇黑腹果蝇突变体hari，影响成年果蝇刚毛机械感觉器官的形成。在这个突变体中，PE插入位点位于编码细胞周期蛋白A（CycA）的基因（CycA）的第一个内含子中。为了在分子水平上分析hari突变体，我们克隆并测序了编码CycA的cDNA和基因组DNA。CycA有七个外显子和六个内含子，其转录单元跨度为6 kb。所有外显子-内含子连接均符合GT/AG共有序列。引物延伸分析和核糖核酸酶保护试验结果表明，CycA有主要和次要转录起始点（tsp）。据我们所知，这是关于多细胞生物CycA基因组序列的首次报道。
