Ueda R, Togashi S, Takahisa M, Tsurumura S, Mikuni M, Kondo K, Miyake T
Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
EMBO J. 1992 Aug;11(8):2935-9. doi: 10.1002/j.1460-2075.1992.tb05363.x.
Cyclin proteins are one of the important components of the mechanism regulating mitosis in eukaryotic cells. We isolated a Drosophila Cyclin-A mutant in which the progenitor cells of the peripheral nervous system (the sensory mother cells) do not divide properly, causing the loss and other abnormalities of mechanosensory organs in the adult fly. Sequence analysis of the mutant genome reveals that a P element is inserted into the first intron of the Cyclin-A gene. A 13 kb wild-type genomic DNA containing the Cyclin-A transcription units rescued the mutant phenotype when introduced into the mutant fly. The regulation of cell type specific expression of the Cyclin-A gene is discussed.
细胞周期蛋白是真核细胞中调节有丝分裂机制的重要组成部分之一。我们分离出一种果蝇细胞周期蛋白A突变体，其中外周神经系统的祖细胞（感觉母细胞）不能正常分裂，导致成年果蝇的机械感觉器官缺失和其他异常。对突变体基因组的序列分析表明，一个P元件插入到细胞周期蛋白A基因的第一个内含子中。当将包含细胞周期蛋白A转录单位的13 kb野生型基因组DNA导入突变果蝇时，挽救了突变体表型。本文还讨论了细胞周期蛋白A基因的细胞类型特异性表达调控。
