DNA复制相关元件（DRE）和DRE结合因子（DREF）对果蝇CycA基因的转录调控。
Transcriptional regulation of the Drosophila CycA gene by the DNA replication-related element (DRE) and DRE binding factor (DREF).
作者信息
Ohno K, Hirose F, Sakaguchi K, Nishida Y, Matsukage A
机构信息
Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan.
出版信息
Nucleic Acids Res. 1996 Oct 15;24(20):3942-6. doi: 10.1093/nar/24.20.3942.
The Drosophila gene for cyclin A is expressed in dividing cells throughout development. This expression pattern is similar to those of genes related to DNA replication, suggesting involvement of some common control mechanism(s). In the upstream region (-71 to -64 with respect to the transcription initiation site) of the CycA gene, we found a sequence identical to the DNA replication-related element (DRE; 5'-TATCGATA), which is important for high level expression of replication-related genes such as those encoding DNA polymerase alpha and proliferating cell nuclear antigen. Transient expression assays with chloramphenicol acetyltransferase (CAT) were carried out to examine the function of the DRE sequence of the CycA gene. Deletion or base substitution mutations resulted in an extensive reduction in CAT expression. Furthermore, monoclonal antibodies against DRE binding factor (DREF) diminished or supershifted the complex of the DREF and DRE-containing fragment. The results indicate that the Drosophila CycA gene is under the control of a DRE/DREF system, as are DNA replication-related genes.
果蝇细胞周期蛋白A基因在整个发育过程中的分裂细胞中表达。这种表达模式与DNA复制相关基因的表达模式相似，表明存在一些共同的调控机制。在细胞周期蛋白A基因的上游区域（相对于转录起始位点为-71至-64），我们发现了一个与DNA复制相关元件（DRE；5'-TATCGATA）相同的序列，该元件对于DNA聚合酶α和增殖细胞核抗原等复制相关基因的高水平表达很重要。利用氯霉素乙酰转移酶（CAT）进行瞬时表达分析，以检测细胞周期蛋白A基因DRE序列的功能。缺失或碱基替代突变导致CAT表达大幅降低。此外，针对DRE结合因子（DREF）的单克隆抗体使DREF与含DRE片段的复合物减少或发生超迁移。结果表明，果蝇细胞周期蛋白A基因如同DNA复制相关基因一样，受DRE/DREF系统的调控。
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