Yano H, Kojiro M, Nakashima T
In Vitro Cell Dev Biol. 1986 Nov;22(11):637-46. doi: 10.1007/BF02623477.
A human hepatocellular carcinoma (HCC) cell line (KYN-1) has been established from a resected HCC of a 58-yr-old Japanese, male patient with HCC. Original resected HCC was moderately differentiated and proliferated in a solid pattern with vague trabecular structure in part. This cell line has been maintained for 10 mo. through 50 passages. Morphological features of KYN-1 cells demonstrated one or more large, round-to-oval nuclei with prominent nucleoli and eosinophilic polygonal-to-spindle abundant cytoplasm. In addition, some of these cells contained mucicarmin-positive materials in the cytoplasm. The cells exhibited a typical epithelial feature with pavementlike cell arrangement, and lacked contact inhibition. The doubling times of the cells grown in a serum-containing and a serum-free medium were about 31 h and 10 to 11 d, respectively. Functionally, KYN-1 cells produced albumin, alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), ferritin, beta 2-microglobulin (BMG), and alpha 1-anti-trypsin (AAT). Positive reactions for albumin, AFP, CEA, and ferritin were identified in the cells by immunohistochemical techniques. Chromosome study revealed the chromosome number in a range from 61 to 74 without mode. The tumorigenicity of KYN-1 cells was identified by the tumor formation after subcutaneous inoculation of the cells into nude mice. The developed tumor showed compact growth of the tumor cells with gland formations containing mucicarmin-positive materials. Features of adenocarcinoma were identified by electron microscopy. The tumor cells were also identified to contain albumin, AFP, CEA, ferritin, and AAT by immunohistochemical techniques. AFP, CEA, and BMG were detected in the sera of nude mice. Thus, KYN-1 cells represented the morphologic features of adenocarcinoma, retaining some characteristics of original HCC. These findings suggest that KYN-1 is a new human HCC cell line with transformation to adenocarcinoma, which will provide useful information to clarify the histogenesis of combined hepatocellular and cholangiocellular carcinoma.
一种人肝癌（HCC）细胞系（KYN-1）是从一名58岁日本男性肝癌患者切除的肝癌组织中建立的。原始切除的肝癌为中度分化，呈实体状增殖，部分区域小梁结构模糊。该细胞系已传代50次，维持了10个月。KYN-1细胞的形态学特征为一个或多个大的、圆形至椭圆形的细胞核，核仁突出，细胞质丰富，呈嗜酸性多边形至梭形。此外，这些细胞中的一些细胞质中含有黏液卡红阳性物质。细胞表现出典型的上皮特征，呈铺路石样细胞排列，且无接触抑制。在含血清和无血清培养基中生长的细胞倍增时间分别约为31小时和10至11天。在功能上，KYN-1细胞产生白蛋白、甲胎蛋白（AFP）、癌胚抗原（CEA）、铁蛋白、β2-微球蛋白（BMG）和α1-抗胰蛋白酶（AAT）。通过免疫组织化学技术在细胞中鉴定出白蛋白、AFP、CEA和铁蛋白的阳性反应。染色体研究显示染色体数在61至74之间，无众数。通过将KYN-1细胞皮下接种到裸鼠体内形成肿瘤，确定了其致瘤性。形成的肿瘤显示肿瘤细胞紧密生长，有含黏液卡红阳性物质的腺体形成。通过电子显微镜鉴定出腺癌特征。通过免疫组织化学技术还鉴定出肿瘤细胞含有白蛋白、AFP、CEA、铁蛋白和AAT。在裸鼠血清中检测到AFP、CEA和BMG。因此，KYN-1细胞代表了腺癌的形态学特征，保留了原始肝癌的一些特征。这些发现表明，KYN-1是一种新的人肝癌细胞系，已转化为腺癌，这将为阐明肝细胞癌和胆管细胞癌合并的组织发生提供有用信息。
