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转录增强哺乳动物细胞内染色体同源重组。

Transcription enhances intrachromosomal homologous recombination in mammalian cells.

作者信息

Nickoloff J A

机构信息

Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.

出版信息

Mol Cell Biol. 1992 Dec;12(12):5311-8. doi: 10.1128/mcb.12.12.5311-5318.1992.

DOI:10.1128/mcb.12.12.5311-5318.1992
PMID:1333040
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360468/
Abstract

The influence of transcription on homologous intrachromosomal recombination between direct and inverted repeats has been examined by using Chinese hamster ovary cells. Recombination was monitored between two integrated neomycin (neo) genes, including one silent allele and a second allele regulated by the inducible mouse mammary tumor virus promoter. Transcription of mouse mammary tumor virus neo alleles was regulated with the glucocorticoid hormone dexamethasone. Alleles transcribed at high levels recombined about two- to sevenfold more frequently than identical alleles transcribed at low levels. Direct repeats recombined primarily by a gene conversion mechanism; inverted repeats produced a variety of rearranged products. These results are discussed in relation to recombinational processes that regulate gene expression, influence gene family structures, and mediate genomic instability associated with cellular transformation and tumorigenesis.

摘要

通过使用中国仓鼠卵巢细胞，研究了转录对直接重复序列和反向重复序列之间同源染色体内重组的影响。监测了两个整合的新霉素（neo）基因之间的重组情况，其中一个是沉默等位基因，另一个等位基因受诱导型小鼠乳腺肿瘤病毒启动子调控。小鼠乳腺肿瘤病毒neo等位基因的转录通过糖皮质激素地塞米松进行调控。高水平转录的等位基因重组频率比低水平转录的相同等位基因高约两到七倍。直接重复序列主要通过基因转换机制进行重组；反向重复序列产生了各种重排产物。结合调节基因表达、影响基因家族结构以及介导与细胞转化和肿瘤发生相关的基因组不稳定性的重组过程，对这些结果进行了讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/232e10f3bafa/molcellb00135-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/5ab0c756a87b/molcellb00135-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/8c02c0e85a43/molcellb00135-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/6aa91602f106/molcellb00135-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/c6235dbad401/molcellb00135-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/c2d3df258d92/molcellb00135-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/232e10f3bafa/molcellb00135-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/5ab0c756a87b/molcellb00135-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/8c02c0e85a43/molcellb00135-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/6aa91602f106/molcellb00135-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/c6235dbad401/molcellb00135-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/c2d3df258d92/molcellb00135-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8dc9/360468/232e10f3bafa/molcellb00135-0046-b.jpg

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