Bratanich A C, Hanson N D, Jones C J
University of Nebraska, Department of Veterinary Sciences, Lincoln 68583-0905.
Virology. 1992 Dec;191(2):988-91. doi: 10.1016/0042-6822(92)90278-w.
Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory neurons of infected animals. Only one virus-encoded latency-related (LR) gene is expressed during a latent infection. The LR transcript overlaps immediate-early transcription unit 1 (IEtu1) and is anti-sense with respect to IEtu1. The transcriptional start site of the LR RNA was mapped to position 724 of the LR gene, downstream from two putative TATA elements. When LR promoter sequences were deleted from a plasmid containing IEtu1 and the LR gene, the resulting construct trans-activated the HSV-1 thymidine kinase (TK) promoter more efficiently than IEtu1 plus the LR gene. Cotransfection of a plasmid containing the intact LR gene with IEtu1 inhibited the ability of IEtu1 to trans-activate the TK promoter. These results imply that a LR gene product(s) repressed the trans-acting capacity of IEtu1.
牛疱疹病毒1型（BHV-1）在受感染动物的感觉神经元中建立潜伏感染。在潜伏感染期间仅表达一种病毒编码的潜伏相关（LR）基因。LR转录本与立即早期转录单元1（IEtu1）重叠，并且相对于IEtu1是反义的。LR RNA的转录起始位点被定位到LR基因的第724位，位于两个假定的TATA元件下游。当从含有IEtu1和LR基因的质粒中缺失LR启动子序列时，所得构建体比IEtu1加LR基因更有效地反式激活HSV-1胸苷激酶（TK）启动子。将含有完整LR基因的质粒与IEtu1共转染抑制了IEtu1反式激活TK启动子的能力。这些结果表明，一种或多种LR基因产物抑制了IEtu1的反式作用能力。
