Kutish G, Mainprize T, Rock D
Department of Veterinary Science, University of Nebraska, Lincoln 68583.
J Virol. 1990 Dec;64(12):5730-7. doi: 10.1128/JVI.64.12.5730-5737.1990.
Approximate 5' and 3' ends of the bovine herpesvirus 1 (BHV-1) latency-related RNA (LR RNA) were mapped in rabbit trigeminal ganglia (TG) by in situ hybridization. The data provide a size estimate of 0.77 to 1.16 kb for the LR RNA. An LR RNA mapping to a similar location was also detected in TG of cattle latently infected with BHV-1. The BHV-1 LR region is transcriptionally active in bovine cell cultures lytically infected with BHV-1. A 1.15-kb transcript, present at early and late times postinfection, of the same sense and approximate size that seen in latently infected TG overlaps a 2.9-kb immediate-early and a 2.6-kb early and late transcription unit present on the complementary strand. Sequence analysis of the LR RNA sense strand indicates the presence of a potential polymerase II promoter in close proximity to the 5' terminus of the LR RNA and two open reading frames within its map positions. The complementary strand contains the 3' portion of a large open reading frame that almost completely overlaps the map position of the LR RNA present on the opposite strand.
通过原位杂交技术，在兔三叉神经节（TG）中绘制了牛疱疹病毒1型（BHV - 1）潜伏相关RNA（LR RNA）的近似5'和3'末端。数据显示LR RNA的大小估计为0.77至1.16 kb。在潜伏感染BHV - 1的牛的TG中也检测到了定位在相似位置的LR RNA。BHV - 1的LR区域在被BHV - 1裂解感染的牛细胞培养物中具有转录活性。在感染后早期和晚期出现的一个1.15 kb转录本，其意义和大小与潜伏感染的TG中所见相似，与互补链上存在的一个2.9 kb立即早期和一个2.6 kb早期及晚期转录单元重叠。LR RNA有义链的序列分析表明，在LR RNA的5'末端附近存在一个潜在的聚合酶II启动子，并且在其图谱位置内有两个开放阅读框。互补链包含一个大的开放阅读框的3'部分，该部分几乎完全与相反链上存在的LR RNA的图谱位置重叠。
