Svenningsson A, Lycke J, Svennerholm B, Gronowitz S, Andersen O
Department of Clinical Chemistry, Sahlgren's Hospital, Gothenburg, Sweden.
Ann Neurol. 1992 Nov;32(5):711-4. doi: 10.1002/ana.410320518.
Polymerase chain reaction analysis was used to investigate the possible role of human spumaretrovirus and oncoretroviruses (human T-cell lymphotropic virus types I [HTLV-I] and II [HTLV-II]) in multiple sclerosis. Eleven patients with relapsing-remitting multiple sclerosis in exacerbation and 11 normal blood donors were included in the study. Cerebrospinal fluid cells, peripheral blood mononuclear cells, and plasma were cocultured with allogeneic mononuclear cells for 6 weeks. Cultured cells were subjected to polymerase chain reaction analysis with primers selected for the pol and gag (human spumaretrovirus), pol and env (HTLV-I), and pol (HTLV-II) genes. Polymerase chain reaction was negative in all patient and blood donor control samples, whereas positive controls were consistently reactive with high sensitivity. No culture exhibited cytopathic effects and supernatants were negative for reverse transcriptase activity. Thus, our results do not support a role for these retroviruses in the pathogenesis of multiple sclerosis.
采用聚合酶链反应分析来研究人泡沫逆转录病毒和致肿瘤逆转录病毒（人T细胞嗜淋巴细胞病毒I型[HTLV-I]和II型[HTLV-II]）在多发性硬化症中可能发挥的作用。该研究纳入了11例处于病情加重期的复发缓解型多发性硬化症患者和11名正常献血者。将脑脊液细胞、外周血单个核细胞和血浆与同种异体单个核细胞共培养6周。用针对pol和gag基因（人泡沫逆转录病毒）、pol和env基因（HTLV-I）以及pol基因（HTLV-II）选择的引物对培养的细胞进行聚合酶链反应分析。在所有患者和献血者对照样本中，聚合酶链反应均为阴性，而阳性对照始终具有高灵敏度的反应性。没有培养物表现出细胞病变效应，并且上清液的逆转录酶活性为阴性。因此，我们的结果不支持这些逆转录病毒在多发性硬化症发病机制中发挥作用。
