Rozenberg F, Lefebvre S, Lubetzki C, Lebon P, Lyon-Caen O, Brahic M, Bureau J F
Unité des Virus Lents, Institut Pasteur, Paris, France.
Ann Neurol. 1991 Mar;29(3):333-6. doi: 10.1002/ana.410290317.
The polymerase chain reaction was used, in a blind study, to look for retroviral sequences in DNA extracted from the peripheral blood mononuclear cells of 11 patients with the spinal form of multiple sclerosis (MS). Control subjects consisted of 7 patients with other neurological diseases and 5 healthy blood donors. Three sets of oligonucleotides were used. They could detect all known human oncoretroviruses, lentiviruses, or spumaretroviruses. The primers recognized conserved sequences in the long terminal repeats of the proviral DNA. Control experiments showed that the primers crossreacted within the human immunodeficiency virus or human T-cell lymphotropic virus group and that they provided the expected level of sensitivity. Therefore the assay could have detected not only known human retroviruses but also new related members. In spite of this, no retroviral sequences were detected in either the MS or the control specimen.
在一项盲法研究中，采用聚合酶链反应在11例脊髓型多发性硬化症（MS）患者外周血单个核细胞提取的DNA中寻找逆转录病毒序列。对照受试者包括7例患有其他神经系统疾病的患者和5名健康献血者。使用了三组寡核苷酸。它们能够检测所有已知的人类嗜肝DNA病毒、慢病毒或泡沫逆转录病毒。这些引物识别前病毒DNA长末端重复序列中的保守序列。对照实验表明，这些引物在人类免疫缺陷病毒或人类嗜T细胞病毒组内会发生交叉反应，并且它们具有预期的灵敏度水平。因此，该检测方法不仅可以检测已知的人类逆转录病毒，还可以检测新的相关成员。尽管如此，在MS患者或对照样本中均未检测到逆转录病毒序列。
