Kyogashima M, Inoue M, Nakayama J, Seto A
Department of Microbiology, Shiga University of Medical Science, Otsu, Japan.
Cancer Lett. 1992 Oct 21;66(3):175-81. doi: 10.1016/0304-3835(92)90245-q.
Blood group H-antigen was clearly demonstrated as glycolipids in Shope carcinoma cells and its culture cell lines by thin-layer chromatography immunostaining and immunohistochemical analyses using the anti-H monoclonal antibody. This antigen was not detected in normal epidermal and Shope papilloma cells of rabbits but was present in the outer sheath cells of hair follicles of normal rabbit skin. The H-antigen was also detected by cytofluorometric analysis on Shope carcinoma cell lines and the cells were lysed in vitro by the antibody and complement in a dose-dependent manner. These results suggested that the H-antigen was aberrantly synthesized in epidermal carcinoma cells.
通过使用抗H单克隆抗体的薄层色谱免疫染色和免疫组织化学分析，血型H抗原在肖普癌细胞及其培养细胞系中被明确证明为糖脂。该抗原在兔的正常表皮和肖普乳头瘤细胞中未检测到，但存在于正常兔皮肤毛囊的外鞘细胞中。通过细胞荧光分析也在肖普癌细胞系中检测到了H抗原，并且这些细胞在体外被抗体和补体以剂量依赖性方式裂解。这些结果表明H抗原在表皮癌细胞中异常合成。
