Kyogashima M, Inoue M, Isono T, Nakayama J, Seto A
Department of Microbiology, Shiga University of Medical Science, Otsu, Japan.
Cancer Lett. 1994 Jun 30;81(2):221-8. doi: 10.1016/0304-3835(94)90206-2.
Monoclonal antibody E6G6 preferentially reacted with well differentiated and non-tumorigenic Shope carcinoma cell lines but hardly reacted with undifferentiated and tumorigenic lines as determined by enzyme-linked immunosorbent assay, cytofluorometry and the complement dependent cytotoxicity test. Immunohistochemical analysis of the primary carcinoma tissue revealed that the antibody intensely stained keratinizing carcinoma cells with marked shrinkage whereas it did not stain normal epidermal keratinizing rabbit cells or benign tumor (Shope papilloma) cells, suggesting that the antigen expression in vivo was closely correlated with terminal differentiation of the carcinoma cells. Thin layer chromatography immunostaining revealed that the antigen was comprised of several neutral glycolipids and that differentiated cells contained about ten times more antigen than undifferentiated cells.
通过酶联免疫吸附测定、细胞荧光测定法和补体依赖性细胞毒性试验确定，单克隆抗体E6G6优先与高分化且无致瘤性的肖普癌细胞系发生反应，但与未分化且具有致瘤性的细胞系几乎不发生反应。对原发性癌组织的免疫组织化学分析显示，该抗体强烈染色具有明显收缩的角化癌细胞，而不染色正常表皮角化兔细胞或良性肿瘤（肖普乳头瘤）细胞，这表明体内抗原表达与癌细胞的终末分化密切相关。薄层色谱免疫染色显示，该抗原由几种中性糖脂组成，分化细胞所含抗原比未分化细胞多约十倍。
